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By Ronald T. Acton

ISBN-10: 0120430509

ISBN-13: 9780120430505

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Development of the muscarinic receptor in reaggre- gates has been followed by specific binding of ^H-guinuclidinyl benzilate (Yamamura and Synder, 1974) to particulate fractions of reaggregate homogenates. The developmental profile of the mus- carinic receptor is similar to that of cholineacetyltransferase activity but somewhat slower in rate of appearance, reaching a maximum expression of receptor after 24 days in culture Haffke, 1976). (Seeds and Thus cholinergic development in these reaggregate cultures reaches maximum levels prior to synaptogenesis; however, since we cannot at present distinguish microscopically the cholinergic synapses, it is possible that cholinergic synapses may be formed first and the slower development reflects other types of synapses.

Crissman, Η. , (1972b), Cancer Res. 32, 2726-2732. Tobey, R. , Ley, K. , (1971), Cancer Res. 31, 46-51. Tobey, R. , Anderson, E. , Petersen, D. , (1967), J. Cell. Physiol. 70, 63-68. Tobey, R. , Gurley, L. , Hildebrand, C. , Ratliff, R. , Walters R. , (1974), in "Control of Proliferation in Animal Cells," (B. Clarkson and R. ), pp. 668-679, Cold Spring Harbor Press, New York. Tobey, R. , Gurley, L. , Hildebrand, C. , Kraemer, P. , Ratliff, R. , Walters, R. , (1975), in "Mammalian Cells: Probes and Problems," (C.

3 ) . (EC and glutamate decar- The greatest increase in these activities occurs during the second week of culture, thus resembling the in situ development in both rate and extent (Seeds, 1 9 7 3 ) . Cultures maintained for as long as three months possess high levels of enzyme activity often surpassing the specific activities found in adult mouse brain. Furthermore, these enzymes appear A G G R E G A T E CULTURES 29 FIGURE 3 Biochemical Differentiation in Aggregate Culture, The development of choline acetyl transferase (a}, acetylcholinesterase (bl , and glutamate decarboxylase Qc) are shown as a function of time in culture.

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Cell Culture and its Application by Ronald T. Acton


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